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Power-Bind™
Streptavidin-coated Particles
We covalently coat our Opti-Link Carboxylate-Modified Particles with Streptavidin.
This results in high biotin-binding capacity and long shelf life.
We have developed a series of non-magnetic Streptavidin coated particles (Power-Bind Streptavidin particles) to improve and simplify the binding of ligands to particles. Power-Bind Streptavidin particles combine the advantages of high surface area and easy, high affinity, high specific activity binding. They can also be used in a variety of diagnostic and molecular biology applications.
Power-Bind Streptavidin-coated particles are monodisperse particle suspensions with Streptavidin covalently bound to the surface (of Opti-Link carboxylate-modified particles) in a highly active form. The use of particles as a solid phase support in various immunoassays and affinity purifications has been known for some time. Achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin particles after simple incubation in buffer. This is due to the high affinity of the biotin-Streptavidin interaction. Power-Bind Streptavidin particles also functions as a spacer, which improves the specific activity of the bound ligand.
Power-Bind Streptavidin particles addresses the concerns of diagnostic test kit manufacturers and nucleic acid researchers. With wellknown biotin-streptavidin reactions, scientists can now easily bind ligands to microparticle surfaces.
Compounds which are difficult to attach to particle surfaces by conventional means may be amenable to biotinylation. This includes compounds for which activation reactions must be performed in organic solvent. In this instance, biotinylation may be carried out in organic solvent; then the biotin derivative is simply mixed with the Power-Bind Streptavidin particles. Nucleic acids, which adsorb poorly to particle surfaces, are readily bound to Power-Bind Streptavidin particles after biotinylation.
Benefits:
- Dissociation constant (Kd 10-15 molar)
- Stably bound ligands
- Easy one-step binding protocols for biotinylated ligands
- High activity of surface bound ligands
- Easily solve difficult coupling problems
- Simple aqueous biotinylation reactions
- Low nonspecific interactions (fully blocked microparticle surface)
- Highly mobile particles for membrane-based applications
- Can be used in EIA formats with biotin/enzyme detection systems
- Beneficial spacer effect of SA molecule
- Choice of 0.3 μm or 0.8 μm diameters for different applications
- High biotin-binding capacity for molecular biology applications
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