Features and Benefits
• No need to weigh individual components – faster and more consistent
• Include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples
• Include clean up tubes for the dispersive solid phase extraction
• Unique shipping and storage rack
• Solvent resistant paper labels aid hassle free sample identification

Fast and efficient clean up of complex samples
ISOLUTE QuEChERS contain a mixture of precisely measured chemical compounds as described in the AOAC and EN standard methods for the analysis of pesticide residues from fruit and vegetable samples. Unlike labor intensive liquid-liquid extraction and complicated solid phase extraction methods, QuEChERS combines clean extracts with rapid work flow and ease of use that ensures reproducibility and faster sample preparation. Biotage’s strict quality control during the manufacturing process ensures consistent performance in an already well defined methodology.

The 15 mL extraction and clean-up tubes contain sufficient sorbent to process samples according to prescribed AOAC and EN methodologies and allow for immediate sample mixing and transfer to larger 50mL sample tubes. All QuEChERS products are shipped in a cardboard rack for ease of use and contain full instructions following the EN and AOAC methodologies.

QuEChERS technique involves two stages:
1. A crude extraction and partitioning step using organic solvent and a salt solution.
2. Followed by further extraction and clean up using dispersive solid phase extraction.

AOAC 2007.01 and European EN 15662 Methodologies
Biotage^® supplies pre-weighed extraction and clean up tubes that conform with AOAC1,2 and the EU3 published methods, including those for waxed and highly pigmented fruit and vegetable samples.

Figure 1	Figure 2

Figure 1 and 2: Pesticide suite was extracted using Biotage QuEChERS products and the relevant methodology (AOAC or EN) for apple (Figure 1 above) and Broccoli (Figure 2 above). In all instances, recoveries were above 75%.

Figure 3	Figure 4

Figure 3 and 4: Pesticide suite was extracted using Biotage QuEChERS products and the relevant methodology (AOAC or EN) for spinach (Figure 3 above) and orange (Figure 4 above). In all instances, recoveries were above 80%.

References
1. AOAC Official Method 2007.01. Pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate.
2. Anastassiades, M., Lehotay, S.J., Stajnbaher, D. and Schenk, F.J. (2003) J AOAC International 86, 412-431.
3. EN 15662. Foods of plant origin- Determination of pesticide residues using GC-MS and /or LC-MS/MS following acetonitrile extraction/partitioning and clean up by dispersive SPE-QuEChERS method.