SKU: BT-17AP Categories: Saporin, Antibodies Product Size: 50 ug | Antibody Type: Polyclonal | Host: Chicken | Reactivity: All species | Conjugate: Biotin | Usage: ELISA, flow cytometry, immunoblotting |
Saporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha2-macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death.
This antibody recognizes native and recombinant saporin. It was developed in chicken using saporin and was purified from chicken egg yolk. It has been conjugated to biotin via an amide bond. The antibody is routinely tested by western blot.
Applications include immunoblotting, ELISA, and flow cytometry.
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